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KMID : 0358419700130030011
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Korean Journal of Obstetrics and Gynecology
1970 Volume.13 No. 3 p.11 ~ p.18
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Immunologic Determination of H.C.G. in Serum of the Patients of Trophoblastic Diseases and Severe Toxemia
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Abstract
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Serum H.C.G. level has been determined in patients with trophoblastic diseases and severe toxemia by the hemagglutination inhibition technic. The results are as follows:
1. A relatively high titer of antiserum was obtained with relatively pure H.C.G. (5,427 I.U./
mg).
2. The specificity of the obtained antiserum has been confirmed by the neutralization test of
biological activity of -H.C.G. on the weight of immature rat ovary.
3. Ouchterlony test for the analysis of the purity of H.C.G. revealed only one clear precipitin
line between antigen and antiserum, indicating that the used H.C.G. contained negligible
amount of other proteins.
4. Serum was treated with acetone and H.C.G. containing fraction was washed with absolute alcohol and ether, and the recovery rate of H.C.G. with the aceton precipitation method was about 95 per cent.
5. In hydatidiform mole, the mean value of H.C.G. in serum was 644.9I.U./ml which was about
10 times higher than the level found in the normal pregnancy at the corresponding duration. 6. The serum concentration of H.C.G. in choriocarcinoma was also remarkably high, which was
about 2-3 times higher than that of normal pregnancy.
7. Inn severe toxemia, H.C.G. concentration in serum revealed considerably high value within the range of uncomplicated pregnancy (t=4.84>2.77 .´% p 0.01).
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